5K97
Flap endonuclease 1 (FEN1) D233N with cleaved product fragment and Sm3+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Flap endonuclease 1 | polymer | 341 | 38510.2 | 1 | UniProt (P39748) Pfam (PF00752) Pfam (PF00867) In PDB | Homo sapiens (Human) | FEN-1,DNase IV,Flap structure-specific endonuclease 1,Maturation factor 1,hFEN-1 |
| 2 | D | DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3') | polymer | 18 | 5476.6 | 1 | synthetic construct | ||
| 3 | E | DNA (5'-D(P*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3') | polymer | 11 | 3438.3 | 1 | synthetic construct | ||
| 4 | F | DNA (5'-D(*AP*CP*CP*GP*TP*CP*C)-3') | polymer | 7 | 2058.4 | 1 | synthetic construct | ||
| 5 | G | DNA (5'-D(P*TP*T)-3') | polymer | 5 | 1479.0 | 1 | synthetic construct | ||
| 6 | A | SAMARIUM (III) ION | non-polymer | 150.4 | 8 | Chemie (SM) | |||
| 7 | A | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 8 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 9 | water | water | 18.0 | 623 | Chemie (HOH) |
Sequence modifications
A: 2 - 336 (UniProt: P39748)
| PDB | External Database | Details |
|---|---|---|
| Asn 233 | Asp 233 | engineered mutation |
| Leu 337 | - | expression tag |
| Glu 338 | - | expression tag |
| Val 339 | - | expression tag |
| Leu 340 | - | expression tag |
| Phe 341 | - | expression tag |
| Gln 342 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 50962.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1304.0 | |
| All* | Total formula weight | 52266.5 |
