5K97

Flap endonuclease 1 (FEN1) D233N with cleaved product fragment and Sm3+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1158
Spacegroup name	P 31 2 1
Unit cell lengths	105.236, 105.236, 104.548
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.849 - 2.102
R-factor	0.1843
Rwork	0.182
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.531
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((dev_2383: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.092	0.071	0.651
Rmeas	0.095
Rpim	0.025
Total number of observations	539312
Number of reflections	39124
<I/σ(I)>	18.4
Completeness [%]	99.5	97.6	97.5
Redundancy	13.8	13.6	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	24% MPEG 2K, 20% KCl, 5% ethylene glycol, 100 mM HEPES pH 7.5