5K78

Dbr1 in complex with 16-mer branched RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	RNA lariat debranching enzyme, putative	polymer	360	41846.9	5	UniProt (C4M1P9) Pfam (PF00149) Pfam (PF20890) In PDB	Entamoeba histolytica
2	X, Y	branch 1 of branched RNA 5'-UACUAA(2'-GUAUGU)CAAGU-3'	polymer	11	3475.1	2		Saccharomyces cerevisiae
3	x, y	branch 2 of branched RNA 5'-UACUAA(2'-GUAUGU)CAAGU-3'	polymer	6	1893.2	2		Saccharomyces cerevisiae
4	E, A, B, C, D	FE (II) ION	non-polymer		55.8	5	Chemie (FE2)
5	E, A, B, C, D	ZINC ION	non-polymer		65.4	5	Chemie (ZN)
6	D, E, A, B, C	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
7		water	water		18.0	25	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 354 (UniProt: C4M1P9)

PDB	External Database	Details
Ala 91	His 91	engineered mutation
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	219971.3
Non-Polymers*	Number of molecules	23
	Total formula weight	1855.1
All*	Total formula weight	221826.3

*Water molecules are not included.