5K64

Crystal structure of VEGF binding IgG1-Fc (Fcab 448)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ig gamma-1 chain C region	polymer	228	25723.3	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (Human)
2	C (C)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
3	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1114.0	1	In PDB GlyTouCan (G64481DJ)
4	E, G, H (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	F (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	I, J (A, B)	water	water		18.0	45	Chemie (HOH)

Sequence modifications

A, B: 225 - 447 (UniProt: P01857)

PDB	External Database	Details
Arg 359	Thr 242	engineered mutation
Phe 360	Lys 243	engineered mutation
Tyr 361	Asn 244	engineered mutation
Asp 388	Glu 271	engineered mutation
Ile 389	Asn 272	engineered mutation
Phe 389	-	insertion
Pro 389	-	insertion
Asn 389	-	insertion
Gly 389	-	insertion
Leu 389	-	insertion
Pro 413	Asp 296	engineered mutation
Tyr 414	Lys 297	engineered mutation
Pro 415	Ser 298	engineered mutation
Ser 416	Arg 299	engineered mutation
Leu 418	Gln 301	engineered mutation
Met 419	Gln 302	engineered mutation
Thr 421	Asn 304	engineered mutation
Arg 422	Val 305	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51446.7
Branched	Number of molecules	2
	Total formula weight	2024.8
Non-Polymers*	Number of molecules	4
	Total formula weight	471.5
All*	Total formula weight	53943.0

*Water molecules are not included.