5K64

Crystal structure of VEGF binding IgG1-Fc (Fcab 448)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-27
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	92.720, 38.860, 97.200
Unit cell angles	90.00, 94.23, 90.00

Refinement procedure

Resolution	46.230 - 2.440
R-factor	0.2174
Rwork	0.215
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jii
RMSD bond length	0.004
RMSD bond angle	0.726
Data reduction software	XDS (XDS v.Oct-2015)
Data scaling software	XDS (XDS v.Oct-2015)
Phasing software	PHENIX (1.10-2247)
Refinement software	PHENIX (1.10-2247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.230	2.527
High resolution limit [Å]	2.440	2.440
Rmerge	0.082	1.620
Number of reflections	26251
<I/σ(I)>	9.12	0.75
Completeness [%]	100.0	100
Redundancy	4	4
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	0.05 M MES, 10% (w/v) PEG 3350