5JWP
Crystal structure of human FIH D201E variant in complex with Zn, alpha-ketoglutarate, and HIF1 alpha peptide.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Hypoxia-inducible factor 1-alpha inhibitor | polymer | 352 | 40624.6 | 1 | UniProt (Q9NWT6) Pfam (PF13621) | Homo sapiens (Human) | Factor inhibiting HIF-1,FIH-1,Hypoxia-inducible factor asparagine hydroxylase |
| 2 | B (B) | Hypoxia-inducible factor 1-alpha | polymer | 19 | 2019.1 | 1 | UniProt (Q16665) Pfam (PF08778) | Homo sapiens | HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,bHLHe78,Member of PAS protein 1,PAS domain-containing protein 8 |
| 3 | C (A) | 2-OXOGLUTARIC ACID | non-polymer | 146.1 | 1 | Chemie (AKG) | |||
| 4 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | F, G, H, I (A) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 7 | J, K (A, B) | water | water | 18.0 | 174 | Chemie (HOH) |
Sequence modifications
A: 1 - 349 (UniProt: Q9NWT6)
B: 788 - 806 (UniProt: Q16665)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Glu 201 | Asp 201 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 800 | Cys 800 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 42643.7 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 687.9 | |
| All* | Total formula weight | 43331.6 |
