5JWP

Crystal structure of human FIH D201E variant in complex with Zn, alpha-ketoglutarate, and HIF1 alpha peptide.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hypoxia-inducible factor 1-alpha inhibitor	polymer	352	40624.6	1	UniProt (Q9NWT6) Pfam (PF13621) In PDB	Homo sapiens (Human)	Factor inhibiting HIF-1,FIH-1,Hypoxia-inducible factor asparagine hydroxylase
2	B	Hypoxia-inducible factor 1-alpha	polymer	19	2019.1	1	UniProt (Q16665) Pfam (PF08778) In PDB	Homo sapiens	HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,bHLHe78,Member of PAS protein 1,PAS domain-containing protein 8
3	A	2-OXOGLUTARIC ACID	non-polymer		146.1	1	Chemie (AKG)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	A	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
7		water	water		18.0	174	Chemie (HOH)

Sequence modifications

A: 1 - 349 (UniProt: Q9NWT6)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Glu 201	Asp 201	engineered mutation

B: 788 - 806 (UniProt: Q16665)

PDB	External Database	Details
Ala 800	Cys 800	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42643.7
Non-Polymers*	Number of molecules	7
	Total formula weight	687.9
All*	Total formula weight	43331.6

*Water molecules are not included.