5JWP
Crystal structure of human FIH D201E variant in complex with Zn, alpha-ketoglutarate, and HIF1 alpha peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.220, 86.220, 149.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.370 - 2.100 |
| R-factor | 0.1727 |
| Rwork | 0.170 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h2l |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.070 | 0.043 | |
| Number of reflections | 33713 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.5 | 95.3 | 99.8 |
| Redundancy | 5.2 | 4.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400 |
