5JJ4
Crystal Structure of a Variant Human Activation-induced Deoxycytidine Deaminase as an MBP fusion protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (C, A, B) | Maltose-binding periplasmic protein,Single-stranded DNA cytosine deaminase | polymer | 546 | 61463.7 | 3 | UniProt (P0AEY0) UniProt (Q9GZX7) Pfam (PF01547) Pfam (PF08210) | Escherichia coli O157:H7 | MBP,MMBP,Maltodextrin-binding protein,Activation-induced cytidine deaminase,Cytidine aminohydrolase |
| 2 | D, E, F (D, E, F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 3 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | alpha-maltotetraose | ||
| 3 | G, I, K (C, A, B) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 4 | H, J, L (C, A, B) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 5 | M, N, O (C, A, B) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
C, A, B: 1 - 367 (UniProt: P0AEY0)
C, A, B: 1023 - 1183 (UniProt: Q9GZX7)
| PDB | External Database | Details |
|---|---|---|
| Ala 359 | Glu 385 | conflict |
| Ala 362 | Lys 388 | conflict |
| Ala 363 | Asp 389 | conflict |
| Asn 367 | Arg 393 | conflict |
| Ala 368 | - | linker |
| Ala 369 | - | linker |
| Ala 370 | - | linker |
| Leu 1005 | - | linker |
| Met 1006 | - | linker |
| Asp 1007 | - | linker |
| Pro 1008 | - | linker |
| His 1009 | - | linker |
| Ile 1010 | - | linker |
| Phe 1011 | - | linker |
| Thr 1012 | - | linker |
| Ser 1013 | - | linker |
| Asn 1014 | - | linker |
| Phe 1015 | - | linker |
| Asn 1016 | - | linker |
| Asn 1017 | - | linker |
| Gly 1018 | - | linker |
| Ile 1019 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| His 1025 | Arg 25 | conflict |
| Lys 1026 | Glu 26 | conflict |
| Glu 1032 | Val 32 | conflict |
| Glu 1034 | Lys 34 | conflict |
| Leu 1036 | Arg 36 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 184391.0 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1999.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 316.5 | |
| All* | Total formula weight | 186707.2 |
