5JJ4
Crystal Structure of a Variant Human Activation-induced Deoxycytidine Deaminase as an MBP fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-25 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.793, 96.806, 108.692 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 35.475 - 2.807 |
| R-factor | 0.1849 |
| Rwork | 0.183 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DM0 and 3EIU |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 52735 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 99.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100mM MES pH 6.5, 200mM Calcium Acetate, 15%PEG8000 |
