5IKN
Crystal Structure of the T7 Replisome in the Absence of DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | DNA-directed DNA polymerase | polymer | 704 | 79703.6 | 3 | UniProt (P00581) Pfam (PF00476) | Enterobacteria phage T7 | DNA polymerase gp5,Gene product 5,Gp5,T7 DNA polymerase |
| 2 | D, E, F, G, H... (D, E, F, G, H...) | DNA primase/helicase | polymer | 486 | 53815.0 | 7 | UniProt (P03692) Pfam (PF21268) Pfam (PF01751) Pfam (PF03796) | Enterobacteria phage T7 | Gene product 4,Gp4 |
| 3 | K, L, M (K, L, M) | Thioredoxin-1 | polymer | 105 | 11414.1 | 3 | UniProt (P0AA27) Pfam (PF00085) | Escherichia coli O157:H7 | Trx-1 |
Sequence modifications
A, B, C: 1 - 704 (UniProt: P00581)
| PDB | External Database | Details |
|---|---|---|
| Ala 5 | Asp 5 | engineered mutation |
| Ala 7 | Glu 7 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 650058.2 | |
| All* | Total formula weight | 650058.2 |
