5IKN

Crystal Structure of the T7 Replisome in the Absence of DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003824	molecular_function	catalytic activity
A	0003887	molecular_function	DNA-directed DNA polymerase activity
A	0004518	molecular_function	nuclease activity
A	0004527	molecular_function	exonuclease activity
A	0004529	molecular_function	DNA exonuclease activity
A	0005515	molecular_function	protein binding
A	0006260	biological_process	DNA replication
A	0006261	biological_process	DNA-templated DNA replication
A	0008152	biological_process	metabolic process
A	0008408	molecular_function	3'-5' exonuclease activity
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0016787	molecular_function	hydrolase activity
A	0039693	biological_process	viral DNA genome replication
A	0046872	molecular_function	metal ion binding
A	0090592	biological_process	DNA synthesis involved in DNA replication
A	0097681	biological_process	double-strand break repair via alternative nonhomologous end joining
B	0000166	molecular_function	nucleotide binding
B	0003676	molecular_function	nucleic acid binding
B	0003677	molecular_function	DNA binding
B	0003824	molecular_function	catalytic activity
B	0003887	molecular_function	DNA-directed DNA polymerase activity
B	0004518	molecular_function	nuclease activity
B	0004527	molecular_function	exonuclease activity
B	0004529	molecular_function	DNA exonuclease activity
B	0005515	molecular_function	protein binding
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0008152	biological_process	metabolic process
B	0008408	molecular_function	3'-5' exonuclease activity
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0016787	molecular_function	hydrolase activity
B	0039693	biological_process	viral DNA genome replication
B	0046872	molecular_function	metal ion binding
B	0090592	biological_process	DNA synthesis involved in DNA replication
B	0097681	biological_process	double-strand break repair via alternative nonhomologous end joining
C	0000166	molecular_function	nucleotide binding
C	0003676	molecular_function	nucleic acid binding
C	0003677	molecular_function	DNA binding
C	0003824	molecular_function	catalytic activity
C	0003887	molecular_function	DNA-directed DNA polymerase activity
C	0004518	molecular_function	nuclease activity
C	0004527	molecular_function	exonuclease activity
C	0004529	molecular_function	DNA exonuclease activity
C	0005515	molecular_function	protein binding
C	0006260	biological_process	DNA replication
C	0006261	biological_process	DNA-templated DNA replication
C	0008152	biological_process	metabolic process
C	0008408	molecular_function	3'-5' exonuclease activity
C	0016740	molecular_function	transferase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0016787	molecular_function	hydrolase activity
C	0039693	biological_process	viral DNA genome replication
C	0046872	molecular_function	metal ion binding
C	0090592	biological_process	DNA synthesis involved in DNA replication
C	0097681	biological_process	double-strand break repair via alternative nonhomologous end joining
D	0003678	molecular_function	DNA helicase activity
D	0003697	molecular_function	single-stranded DNA binding
D	0003896	molecular_function	DNA primase activity
D	0005524	molecular_function	ATP binding
D	0006260	biological_process	DNA replication
D	0043139	molecular_function	5'-3' DNA helicase activity
E	0003678	molecular_function	DNA helicase activity
E	0003697	molecular_function	single-stranded DNA binding
E	0003896	molecular_function	DNA primase activity
E	0005524	molecular_function	ATP binding
E	0006260	biological_process	DNA replication
E	0043139	molecular_function	5'-3' DNA helicase activity
F	0003678	molecular_function	DNA helicase activity
F	0003697	molecular_function	single-stranded DNA binding
F	0003896	molecular_function	DNA primase activity
F	0005524	molecular_function	ATP binding
F	0006260	biological_process	DNA replication
F	0043139	molecular_function	5'-3' DNA helicase activity
G	0003678	molecular_function	DNA helicase activity
G	0003697	molecular_function	single-stranded DNA binding
G	0003896	molecular_function	DNA primase activity
G	0005524	molecular_function	ATP binding
G	0006260	biological_process	DNA replication
G	0043139	molecular_function	5'-3' DNA helicase activity
H	0003678	molecular_function	DNA helicase activity
H	0003697	molecular_function	single-stranded DNA binding
H	0003896	molecular_function	DNA primase activity
H	0005524	molecular_function	ATP binding
H	0006260	biological_process	DNA replication
H	0043139	molecular_function	5'-3' DNA helicase activity
I	0003678	molecular_function	DNA helicase activity
I	0003697	molecular_function	single-stranded DNA binding
I	0003896	molecular_function	DNA primase activity
I	0005524	molecular_function	ATP binding
I	0006260	biological_process	DNA replication
I	0043139	molecular_function	5'-3' DNA helicase activity
J	0003678	molecular_function	DNA helicase activity
J	0003697	molecular_function	single-stranded DNA binding
J	0003896	molecular_function	DNA primase activity
J	0005524	molecular_function	ATP binding
J	0006260	biological_process	DNA replication
J	0043139	molecular_function	5'-3' DNA helicase activity
K	0005829	cellular_component	cytosol
K	0015035	molecular_function	protein-disulfide reductase activity
K	0015036	molecular_function	disulfide oxidoreductase activity
K	0045454	biological_process	cell redox homeostasis
L	0005829	cellular_component	cytosol
L	0015035	molecular_function	protein-disulfide reductase activity
L	0015036	molecular_function	disulfide oxidoreductase activity
L	0045454	biological_process	cell redox homeostasis
M	0005829	cellular_component	cytosol
M	0015035	molecular_function	protein-disulfide reductase activity
M	0015036	molecular_function	disulfide oxidoreductase activity
M	0045454	biological_process	cell redox homeostasis

Functional Information from PROSITE/UniProt

site_id	PS00194
Number of Residues	19
Details	THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL

Chain	Residue	Details
K	LEU24-LEU42

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site_id	PS00447
Number of Residues	20
Details	DNA_POLYMERASE_A DNA polymerase family A signature. RdnAKtfiYGflYgaGdekI

Chain	Residue	Details
A	ARG518-ILE537

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Nucleophile => ECO:0000250

Chain	Residue	Details
K	CYS32
H	ASP207
I	GLU157
I	ASP207
J	GLU157
J	ASP207
K	CYS35
L	CYS32
L	CYS35
M	CYS32
M	CYS35
G	GLU157
G	ASP207
H	GLU157

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	SITE: Deprotonates C-terminal active site Cys => ECO:0000250

Chain	Residue	Details
K	ASP26
L	ASP26
M	ASP26
G	ASP237
H	ASP237
I	ASP237
J	ASP237
C	ALA476
C	ASP654

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	SITE: Contributes to redox potential value => ECO:0000250

Chain	Residue	Details
K	GLY33
C	ARG518
C	LYS522
C	TYR526
K	PRO34
L	GLY33
L	PRO34
M	GLY33
M	PRO34
J	SER312
B	TYR526
C	HIS506

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: N6-acetyllysine => ECO:0000250

Chain	Residue	Details
K	LYS69
L	LYS69
M	LYS69
G	ARG361
H	ARG361
I	ARG361
J	ARG361

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site_id	SWS_FT_FI5
Number of Residues	7
Details	SITE: dTTP/dATP binding => ECO:0000255|HAMAP-Rule:MF_04154, ECO:0000305|PubMed:10535735

Chain	Residue	Details
D	HIS465
E	HIS465
F	HIS465
G	HIS465
H	HIS465
I	HIS465
J	HIS465

---

site_id	SWS_FT_FI6
Number of Residues	21
Details	SITE: dTTP/dATP binding => ECO:0000255|HAMAP-Rule:MF_04154, ECO:0000305|PubMed:10535735, ECO:0000305|PubMed:10892646

Chain	Residue	Details
D	ARG504
G	ARG504
G	ARG522
G	TYR535
H	ARG504
H	ARG522
H	TYR535
I	ARG504
I	ARG522
I	TYR535
J	ARG504
D	ARG522
J	ARG522
J	TYR535
D	TYR535
E	ARG504
E	ARG522
E	TYR535
F	ARG504
F	ARG522
F	TYR535

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