5IKN
Crystal Structure of the T7 Replisome in the Absence of DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 174.866, 238.091, 243.466 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.983 | 4.880 |
High resolution limit [Å] | 4.800 | 4.800 |
Number of reflections | 41183 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 97.9 | 97.6 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 1.47-1.62M Sodium Formate 0.1M Tris pH 7.5 |