5I2D

Crystal structure of T. thermophilus TTHB099 class II transcription activation complex: TAP-RPo

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, L, M (A, B, L, M)	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	4	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, N (C, N)	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	2	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, O (D, O)	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	2	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, P (E, P)	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	2	UniProt (Q8RQE7) Pfam (PF01192)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, Q (F, Q)	RNA polymerase sigma factor SigA	polymer	443	50769.4	2	UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
6	G, H, R, S (G, H, R, S)	Transcriptional regulator, Crp family	polymer	215	24344.8	4	UniProt (Q53W63) Pfam (PF00027) Pfam (PF13545)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
7	I, T (I, T)	DNA (72-MER)	polymer	72	22234.2	2		Thermus thermophilus
8	J, U (J, U)	DNA (72-MER)	polymer	72	22341.3	2		Thermus thermophilus
9	K, V (K, V)	RNA (5'-R(*UP*CP*GP*A)-3')	polymer	4	1240.8	2		Thermus thermophilus
10	AA, W, X, Z (O, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
11	BA, Y (O, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

F, Q: 1 - 423 (UniProt: Q5SKW1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

G, H, R, S: 1 - 195 (UniProt: Q53W63)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	1046709.5
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	1047019.7

*Water molecules are not included.