5I2D
Crystal structure of T. thermophilus TTHB099 class II transcription activation complex: TAP-RPo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 171.487, 105.446, 374.585 |
Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
Resolution | 49.310 - 4.405 |
R-factor | 0.2425 |
Rwork | 0.241 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g7h |
RMSD bond length | 0.003 |
RMSD bond angle | 0.662 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.310 | 4.480 |
High resolution limit [Å] | 4.400 | 4.400 |
Rmerge | 0.187 | |
Number of reflections | 71660 | |
<I/σ(I)> | 8.182 | |
Completeness [%] | 98.7 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 295 | 0.1 M HEPES, pH 7.6, 0.1 M MgCl2, and 6% PEG4000 |