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5I2D

Crystal structure of T. thermophilus TTHB099 class II transcription activation complex: TAP-RPo

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyPIXEL
Collection date2013-04-26
DetectorPSI PILATUS 6M
Wavelength(s)1.1
Spacegroup nameP 1 21 1
Unit cell lengths171.487, 105.446, 374.585
Unit cell angles90.00, 102.39, 90.00
Refinement procedure
Resolution49.310 - 4.405
R-factor0.2425
Rwork0.241
R-free0.28400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4g7h
RMSD bond length0.003
RMSD bond angle0.662
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwarePHENIX (1.8_1069)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.3104.480
High resolution limit [Å]4.4004.400
Rmerge0.187
Number of reflections71660
<I/σ(I)>8.182
Completeness [%]98.7
Redundancy5.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.62950.1 M HEPES, pH 7.6, 0.1 M MgCl2, and 6% PEG4000

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