5I08

Prefusion structure of a human coronavirus spike protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein,Foldon chimera	polymer	1299	144295.9	3	UniProt (Q0ZME7) UniProt (P10104) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) Pfam (PF07921) UniProt (by SIFTS) (M1E1E4)	Human coronavirus HKU1 (isolate N5) (HCoV-HKU1)	HKU1 prefusion spike protein, S glycoprotein, E2, Peplomer protein,Fibritin, Collar protein,Whisker antigen control protein

Sequence modifications

A, B, C: 14 - 1276 (UniProt: Q0ZME7)

PDB	External Database	Details
Gly 752	Arg 752	engineered mutation
Gly 753	Arg 753	engineered mutation
Ser 754	Lys 754	engineered mutation
Gly 755	Arg 755	engineered mutation
Ser 756	Arg 756	engineered mutation
Gly 1277	-	linker
Ser 1278	-	linker

A, B, C: 1279 - 1305 (UniProt: P10104)

PDB	External Database	Details
Leu 1300	Phe 479	conflict
Gly 1306	-	expression tag
Leu 1307	-	expression tag
Glu 1308	-	expression tag
Val 1309	-	expression tag
Leu 1310	-	expression tag
Phe 1311	-	expression tag
Gln 1312	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	432887.7
All*	Total formula weight	432887.7

*Water molecules are not included.