5I08
Prefusion structure of a human coronavirus spike protein
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Staining material | uranyl formate |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.04 |
| Number of Particle | 39164 |
| Software | DoG Picker,Coot,RELION,IMAGIC,Rosetta,FindEM,Leginon,CTFFIND3,MODELLER |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
