5HZH
Crystal structure of photoinhibitable Rac1 containing C450A mutant LOV2 domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ras-related C3 botulinum toxin substrate 1,NPH1-1,Ras-related C3 botulinum toxin substrate 1 | polymer | 332 | 37066.3 | 1 | UniProt (P63000) UniProt (O49003) Pfam (PF13426) Pfam (PF00071) | Homo sapiens (Human) | Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1,Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1 |
| 2 | B (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 3 | C (A) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E, F (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | G (A) | water | water | 18.0 | 27 | Chemie (HOH) |
Sequence modifications
A: 1 - 47 (UniProt: P63000)
A: 51 - 193 (UniProt: O49003)
A: 197 - 329 (UniProt: P63000)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 97 | Cys 450 | engineered mutation |
| Gly 194 | - | linker |
| Ser 195 | - | linker |
| Gly 196 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 48 | - | linker |
| Ser 49 | - | linker |
| Gly 50 | - | linker |
| Leu 210 | Gln 61 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 37066.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1084.0 | |
| All* | Total formula weight | 38150.2 |
