5HZH

Crystal structure of photoinhibitable Rac1 containing C450A mutant LOV2 domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ras-related C3 botulinum toxin substrate 1,NPH1-1,Ras-related C3 botulinum toxin substrate 1	polymer	332	37066.3	1	UniProt (P63000) UniProt (O49003) Pfam (PF13426) Pfam (PF00071) In PDB	Homo sapiens (Human)	Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1,Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1
2	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
3	A	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6		water	water		18.0	27	Chemie (HOH)

Sequence modifications

A: 1 - 47 (UniProt: P63000)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

A: 51 - 193 (UniProt: O49003)

PDB	External Database	Details
Ala 97	Cys 450	engineered mutation
Gly 194	-	linker
Ser 195	-	linker
Gly 196	-	linker

A: 197 - 329 (UniProt: P63000)

PDB	External Database	Details
Gly 48	-	linker
Ser 49	-	linker
Gly 50	-	linker
Leu 210	Gln 61	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37066.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1084.0
All*	Total formula weight	38150.2

*Water molecules are not included.