5HZH
Crystal structure of photoinhibitable Rac1 containing C450A mutant LOV2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97885 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.050, 74.270, 58.400 |
| Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
| Resolution | 37.135 - 2.600 |
| R-factor | 0.2063 |
| Rwork | 0.204 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wkq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.101 | 0.685 |
| Number of reflections | 9516 | |
| <I/σ(I)> | 14.3 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M calcium acetate, 26% (w/v) PEG 3350 |
