5HPT

System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: WWP1, Ubv P2.3 and UBCH7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G (A, D, G)	NEDD4-like E3 ubiquitin-protein ligase WWP1	polymer	383	45134.6	3	UniProt (Q9H0M0) Pfam (PF00632)	Homo sapiens (Human)	Atrophin-1-interacting protein 5,AIP5,TGIF-interacting ubiquitin ligase 1,Tiul1,WW domain-containing protein 1
2	B, E, H (B, E, H)	Ubiquitin variant P2.3	polymer	83	9368.4	3	Pfam (PF00240) UniProt (by SIFTS) (P62987)	Homo sapiens (Human)
3	C, F (C, F)	Ubiquitin-conjugating enzyme E2 L3	polymer	161	18701.4	2	UniProt (P68036) Pfam (PF00179)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3
4	I, J, K (A, C, D)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, D, G: 537 - 917 (UniProt: Q9H0M0)

PDB	External Database	Details
Gly 535	-	expression tag
Ser 536	-	expression tag

C, F: 2 - 154 (UniProt: P68036)

PDB	External Database	Details
Gly 155	-	expression tag
Gly 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	200911.9
All*	Total formula weight	200911.9

*Water molecules are not included.