Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5HPT

System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: WWP1, Ubv P2.3 and UBCH7

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004842	molecular_function	ubiquitin-protein transferase activity
C	0000151	cellular_component	ubiquitin ligase complex
C	0000209	biological_process	protein polyubiquitination
C	0003713	molecular_function	transcription coactivator activity
C	0003723	molecular_function	RNA binding
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0008283	biological_process	cell population proliferation
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0019787	molecular_function	ubiquitin-like protein transferase activity
C	0019899	molecular_function	enzyme binding
C	0031398	biological_process	positive regulation of protein ubiquitination
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032446	biological_process	protein modification by small protein conjugation
C	0036211	biological_process	protein modification process
C	0044770	biological_process	cell cycle phase transition
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0070979	biological_process	protein K11-linked ubiquitination
C	0071383	biological_process	cellular response to steroid hormone stimulus
C	0071385	biological_process	cellular response to glucocorticoid stimulus
C	0097027	molecular_function	ubiquitin-protein transferase activator activity
C	0098793	cellular_component	presynapse
C	1903955	biological_process	positive regulation of protein targeting to mitochondrion
D	0004842	molecular_function	ubiquitin-protein transferase activity
F	0000151	cellular_component	ubiquitin ligase complex
F	0000209	biological_process	protein polyubiquitination
F	0003713	molecular_function	transcription coactivator activity
F	0003723	molecular_function	RNA binding
F	0004842	molecular_function	ubiquitin-protein transferase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006355	biological_process	regulation of DNA-templated transcription
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0008283	biological_process	cell population proliferation
F	0016567	biological_process	protein ubiquitination
F	0016740	molecular_function	transferase activity
F	0019787	molecular_function	ubiquitin-like protein transferase activity
F	0019899	molecular_function	enzyme binding
F	0031398	biological_process	positive regulation of protein ubiquitination
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0032446	biological_process	protein modification by small protein conjugation
F	0036211	biological_process	protein modification process
F	0044770	biological_process	cell cycle phase transition
F	0045893	biological_process	positive regulation of DNA-templated transcription
F	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
F	0061631	molecular_function	ubiquitin conjugating enzyme activity
F	0070979	biological_process	protein K11-linked ubiquitination
F	0071383	biological_process	cellular response to steroid hormone stimulus
F	0071385	biological_process	cellular response to glucocorticoid stimulus
F	0097027	molecular_function	ubiquitin-protein transferase activator activity
F	0098793	cellular_component	presynapse
F	1903955	biological_process	positive regulation of protein targeting to mitochondrion
G	0004842	molecular_function	ubiquitin-protein transferase activity

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNIdek.GqVCLpvI

Chain	Residue	Details
C	TYR75-ILE90

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
B	LYS27-ASP52

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
B	ARG54
B	ARG72
E	ARG54
E	ARG72
H	ARG54
H	ARG72

site_id	SWS_FT_FI2
Number of Residues	3
Details	SITE: Essential for function

Chain	Residue	Details
B	TYR68
E	TYR68
H	TYR68

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269\|PubMed:24660806, ECO:0000269\|PubMed:24751536, ECO:0000269\|PubMed:24784582, ECO:0000269\|PubMed:25474007, ECO:0000269\|PubMed:25527291

Chain	Residue	Details
B	SER65
E	SER65
H	SER65

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269\|PubMed:32330457

Chain	Residue	Details
B	SER66
E	SER66
H	SER66

site_id	SWS_FT_FI5
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
B	LYS6
E	LYS6
H	LYS6

site_id	SWS_FT_FI6
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106

Chain	Residue	Details
B	MSE63
E	MSE63
H	MSE63

site_id	SWS_FT_FI7
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144

Chain	Residue	Details
B	LYS11
B	LYS48
E	LYS11
E	LYS48
H	LYS11
H	LYS48

site_id	SWS_FT_FI8
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860

Chain	Residue	Details
B	LYS27
E	LYS27
H	LYS27

site_id	SWS_FT_FI9
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127

Chain	Residue	Details
B	LYS29
E	LYS29
H	LYS29

site_id	SWS_FT_FI10
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577

Chain	Residue	Details
B	LYS33
E	LYS33
H	LYS33

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
C	CYS86	nucleofuge

site_id	MCSA2
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
F	CYS86	nucleofuge

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)