5HPT

System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: WWP1, Ubv P2.3 and UBCH7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	114.005, 118.897, 158.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.146 - 2.840
R-factor	0.219
Rwork	0.218
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nd7
RMSD bond length	0.011
RMSD bond angle	1.612
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	95.150
High resolution limit [Å]	2.840
Number of reflections	97828
<I/σ(I)>	15.7
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	isopropanol 10%, PEG3350 8%, sodium citrate 0.1 M