5HJO

Murine endoplasmic reticulum alpha-glucosidase II with bound substrate analogue

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Neutral alpha-glucosidase AB	polymer	857	97894.6	2	UniProt (Q8BHN3) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365)	Mus musculus (Mouse)	Alpha-glucosidase 2,Glucosidase II subunit alpha
2	B, D (B, D)	Glucosidase 2 subunit beta	polymer	83	8912.6	2	UniProt (O08795) Pfam (PF12999)	Mus musculus (Mouse)	80K-H protein,Glucosidase II subunit beta,Protein kinase C substrate 60.1 kDa protein heavy chain,PKCSH
3	E, F (E, F)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G81315DD)
4	G, H (G, H)	2-deoxy-alpha-D-arabino-hexopyranose-(1-3)-D-glucal	branched		292.3	2	In PDB GlyTouCan (G58893KD)
5	I, X (A, C)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)	Mus musculus
6	J, Y (A, C)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)	Mus musculus
7	AA, BA, CA, DA, EA... (C, A)	FORMIC ACID	non-polymer		46.0	20	Chemie (FMT)	Mus musculus
8	JA, U (D, B)	D-glucal	non-polymer		146.1	2	Chemie (DGO)
9	KA, LA, V, W (D, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
10	MA, NA, OA, PA (A, B, C, D)	water	water		18.0	648	Chemie (HOH)

Sequence modifications

A, C: 33 - 944 (UniProt: Q8BHN3)

PDB	External Database	Details
-	Val 186	deletion
-	Pro 187	deletion
-	Phe 188	deletion
-	Ser 189	deletion
-	Asp 190	deletion
-	Lys 191	deletion
-	Val 192	deletion
-	Ser 193	deletion
-	Leu 194	deletion
-	Ala 195	deletion
-	Leu 196	deletion
-	Gly 197	deletion
-	Ser 198	deletion
-	Val 199	deletion
-	Trp 200	deletion
-	Asp 201	deletion
-	Lys 202	deletion
-	Ile 203	deletion
-	Lys 204	deletion
-	Asn 205	deletion
-	Leu 206	deletion
-	Phe 207	deletion
-	Ser 208	deletion
-	Arg 209	deletion
-	Gln 210	deletion
-	Glu 211	deletion
-	Ser 212	deletion
-	Lys 213	deletion
-	Asp 214	deletion
-	Pro 215	deletion
-	Ala 216	deletion
-	Glu 217	deletion
-	Gly 218	deletion
-	Asn 219	deletion
-	Gly 220	deletion
-	Ala 221	deletion
-	Gln 222	deletion
-	Pro 223	deletion
-	Glu 224	deletion
-	Ala 225	deletion
-	Thr 226	deletion
-	Pro 227	deletion
-	Gly 228	deletion
-	Asp 229	deletion
-	Gly 230	deletion
-	Asp 231	deletion
-	Lys 232	deletion
-	Pro 233	deletion
-	Glu 234	deletion
-	Glu 235	deletion
-	Thr 236	deletion
-	Gln 237	deletion
-	Glu 238	deletion
-	Lys 239	deletion
-	Ala 240	deletion
-	Glu 241	deletion
-	Lys 242	deletion
-	Asp 243	deletion
-	Thr 351	deletion
-	Ala 352	deletion
-	Gly 353	deletion
-	Lys 354	deletion
-	Thr 355	deletion
-	Leu 356	deletion
-	Phe 357	deletion
-	Gly 358	deletion
-	Lys 359	deletion
-	Met 360	deletion
-	Leu 361	deletion
-	Asp 362	deletion
-	Tyr 363	deletion
-	Leu 364	deletion
-	Gln 365	deletion
-	Gly 366	deletion
-	Ser 367	deletion
-	Gly 368	deletion
-	Glu 369	deletion
Thr 945	-	expression tag
Ser 946	-	expression tag
Val 947	-	expression tag
Leu 948	-	expression tag
Ile 949	-	expression tag
Leu 950	-	expression tag
Arg 951	-	expression tag
Lys 952	-	expression tag
Pro 953	-	expression tag
Gly 954	-	expression tag
Val 955	-	expression tag
Ser 956	-	expression tag
Val 957	-	expression tag
Ala 958	-	expression tag
Ser 959	-	expression tag
Asp 960	-	expression tag
Trp 961	-	expression tag
Ser 962	-	expression tag
Ile 963	-	expression tag
His 964	-	expression tag
Leu 965	-	expression tag
Arg 966	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	213614.5
Branched	Number of molecules	4
	Total formula weight	2081.9
Non-Polymers*	Number of molecules	30
	Total formula weight	1615.3
All*	Total formula weight	217311.8

*Water molecules are not included.