5HJO

Murine endoplasmic reticulum alpha-glucosidase II with bound substrate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	127.050, 176.440, 103.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.100 - 2.290
R-factor	0.206
Rwork	0.204
R-free	0.23600
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.100	2.297
High resolution limit [Å]	2.290	2.290
Number of reflections	101815
<I/σ(I)>	8.7	1.1
Completeness [%]	96.9	70.6
Redundancy	6.5	5
CC(1/2)		0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	291	30 % Morpheus ethylene glycol/PEG 8000 mix, 0.1 M Morpheus carboxylic acids mix, 0.1 M Morpheus buffer system 1