5H9E
Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target (32-nt spacer) at 3.20 angstrom resolution.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR system Cascade subunit CasA | polymer | 502 | 55965.0 | 1 | UniProt (Q46901) Pfam (PF09481) | Escherichia coli (strain K12) | CRISPR type I-E/Ecoli-associated protein CasA/Cse1,CRISPR-associated protein CasA/Cse1 |
| 2 | B, C (B, C) | CRISPR system Cascade subunit CasB | polymer | 182 | 21207.3 | 2 | UniProt (P76632) Pfam (PF09485) | Escherichia coli (strain K12) | |
| 3 | D, E, F, G, H... (D, E, F, G, H...) | CRISPR system Cascade subunit CasC | polymer | 363 | 40071.4 | 6 | UniProt (Q46899) Pfam (PF09344) | Escherichia coli (strain K12) | |
| 4 | J (J) | CRISPR system Cascade subunit CasD | polymer | 224 | 25239.8 | 1 | UniProt (Q46898) Pfam (PF09704) | Escherichia coli (strain K12) | |
| 5 | K (L) | crRNA (61-MER) | polymer | 61 | 19677.6 | 1 | Escherichia coli | ||
| 6 | L (M) | DNA (47-MER) Non-target | polymer | 47 | 14447.3 | 1 | Escherichia coli | ||
| 7 | M (N) | DNA (47-MER) Target | polymer | 47 | 14434.3 | 1 | Escherichia coli | ||
| 8 | N (K) | CRISPR system Cascade subunit CasE | polymer | 199 | 22324.0 | 1 | UniProt (Q46897) Pfam (PF08798) | Escherichia coli (strain K12) | CasE endoRNase,crRNA endonuclease |
| 9 | O (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
B, C: 1 - 160 (UniProt: P76632)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Ser -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Ala -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Tyr -1 | - | expression tag |
| Gln 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 434930.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 434996.3 |
