5H9E

Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target (32-nt spacer) at 3.20 angstrom resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	92.807, 149.825, 404.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.085 - 3.210
R-factor	0.21
Rwork	0.209
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h9f
RMSD bond length	0.005
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.085
High resolution limit [Å]	3.200
Rmerge	0.265
Number of reflections	90280
<I/σ(I)>	5.8
Completeness [%]	96.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291.95	1.6 M Na/K Phosphate