5GZK

Endo-beta-1,2-glucanase from Chitinophaga pinensis - sophorotriose and glucose complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Uncharacterized protein	polymer	461	52392.6	2	UniProt (C7PIC2)	Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034)
2	C, D (C, D)	beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose	branched		504.4	2	In PDB GlyTouCan (G74665DT)
3	E, J (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	F, K (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	G, M (A, B)	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
6	H (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
7	I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8	L (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	N (B)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
10	O, P (A, B)	water	water		18.0	666	Chemie (HOH)

Sequence modifications

A, B: 1 - 441 (UniProt: C7PIC2)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	104785.3
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1202.5
All*	Total formula weight	106996.7

*Water molecules are not included.