5GZK
Endo-beta-1,2-glucanase from Chitinophaga pinensis - sophorotriose and glucose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.106, 117.401, 75.554 |
Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
Resolution | 49.460 - 1.700 |
R-factor | 0.16761 |
Rwork | 0.166 |
R-free | 0.19875 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gzh |
RMSD bond length | 0.025 |
RMSD bond angle | 2.158 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.110 | 0.680 |
Number of reflections | 120399 | |
<I/σ(I)> | 13.1 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 | 3.6 |
CC(1/2) | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 0.2 M sodium chloride, 0.1 M sodium/potassium phosphate (pH 6.2), 10% PEG 8000 |