5GWT

4-hydroxyisoleucine dehydrogenase mutant complexed with NADH and succinate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	4-hydroxyisolecuine dehydrogenase	polymer	278	30796.7	4	UniProt (A0A0K0Q8K4)	Bacillus thuringiensis
2	E, G, J, L (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	F, H, I, K (A, B, C, D)	SUCCINIC ACID	non-polymer		118.1	4	Chemie (SIN)
4	M, N, O, P (A, B, C, D)	water	water		18.0	383	Chemie (HOH)

Sequence modifications

A, B, C, D: 5 - 248 (UniProt: A0A0K0Q8K4)

PDB	External Database	Details
Lys 144	Glu 144	engineered mutation
Gln 242	Trp 242	engineered mutation
Leu 249	-	expression tag
Glu 250	-	expression tag
Lys 251	-	expression tag
Gly 252	-	expression tag
Glu 253	-	expression tag
Leu 254	-	expression tag
Asn 255	-	expression tag
Ser 256	-	expression tag
Lys 257	-	expression tag
Leu 258	-	expression tag
Glu 259	-	expression tag
Gly 260	-	expression tag
Lys 261	-	expression tag
Pro 262	-	expression tag
Ile 263	-	expression tag
Pro 264	-	expression tag
Asn 265	-	expression tag
Pro 266	-	expression tag
Leu 267	-	expression tag
Leu 268	-	expression tag
Gly 269	-	expression tag
Leu 270	-	expression tag
Asp 271	-	expression tag
Ser 272	-	expression tag
Thr 273	-	expression tag
Arg 274	-	expression tag
Thr 275	-	expression tag
Gly 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	123186.6
Non-Polymers*	Number of molecules	8
	Total formula weight	3126.1
All*	Total formula weight	126312.7

*Water molecules are not included.