5GWT
4-hydroxyisoleucine dehydrogenase mutant complexed with NADH and succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-10-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | H 3 |
| Unit cell lengths | 231.846, 231.846, 78.957 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 1.900 |
| R-factor | 0.17493 |
| Rwork | 0.174 |
| R-free | 0.20058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9i |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.103 | |
| Number of reflections | 124974 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8 M succinate, pH 7.0 |
