5GW7
Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose
Replaces: 5CA4Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Glucosidase YgjK | polymer | 760 | 85946.8 | 2 | UniProt (P42592) Pfam (PF21152) Pfam (PF22422) In PDB | Escherichia coli K-12 | |
2 | C, D | beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900008) GlyTouCan (G88362QR) | alpha-lactose | ||
3 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A, B | beta-D-glucopyranose | non-polymer | 180.2 | 2 | Chemie (BGC) | |||
6 | water | water | 18.0 | 688 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 760 (UniProt: P42592)
PDB | External Database | Details |
---|---|---|
Ala 727 | Glu 750 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 171893.6 | |
Branched | Number of molecules | 2 |
Total formula weight | 684.6 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 489.1 | |
All* | Total formula weight | 173067.2 |