5GW7

Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose

Replaces:  5CA4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glucosidase YgjK	polymer	760	85946.8	2	UniProt (P42592) Pfam (PF21152) Pfam (PF22422)	Escherichia coli K-12
2	C, D (C, D)	beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900008) GlyTouCan (G88362QR)		alpha-lactose
3	E, H (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	F, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G, J (A, B)	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
6	K, L (A, B)	water	water		18.0	688	Chemie (HOH)

Sequence modifications

A, B: 1 - 760 (UniProt: P42592)

PDB	External Database	Details
Ala 727	Glu 750	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	171893.6
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	6
	Total formula weight	489.1
All*	Total formula weight	173067.2

*Water molecules are not included.