5GW7

Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose

Replaces:  5CA4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	56.740, 137.069, 81.599
Unit cell angles	90.00, 100.00, 90.00

Refinement procedure

Resolution	47.090 - 2.200
R-factor	0.15693
Rwork	0.154
R-free	0.20847
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w7t
RMSD bond length	0.009
RMSD bond angle	1.304
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.084	0.297
Number of reflections	60457
<I/σ(I)>	21.9	6
Completeness [%]	97.3	91.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12% PEG 8000, 0.4 M magnesium chloride, 100 mM Tris-HCl buffer