5GKP

Crystal structure of the EndoG worm homologue CPS-6 H148A/F122A in complex with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endonuclease G, mitochondrial	polymer	252	28697.7	2	UniProt (Q95NM6) Pfam (PF01223)	Caenorhabditis elegans	Endo G,Ced-3 protease suppressor 6
2	C, D (C, D)	DNA (5'-D(*TP*TP*TP*TP*T)-3')	polymer	8	2413.6	2		synthetic construct
3	E, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H, I, J (A, B, C, D)	water	water		18.0	175	Chemie (HOH)

Sequence modifications

A, B: 63 - 305 (UniProt: Q95NM6)

PDB	External Database	Details
Met 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
Gly 61	-	expression tag
Ser 62	-	expression tag
Ala 122	Phe 122	engineered mutation
Ala 148	His 148	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	62222.6
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	62271.2

*Water molecules are not included.