5GKP

Crystal structure of the EndoG worm homologue CPS-6 H148A/F122A in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	193
Detector technology	CCD
Collection date	2014-01-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	56.672, 72.562, 127.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.783 - 2.300
R-factor	0.1801
Rwork	0.173
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s5b
RMSD bond length	0.014
RMSD bond angle	1.426
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.350
High resolution limit [Å]	2.300	2.270
Number of reflections	25101
<I/σ(I)>	23.1
Completeness [%]	99.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	279	0.1 M HEPES, 30% PEG 1000