5GAN
The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | V | U4 snRNA | polymer | 160 | 51186.0 | 1 | GenBank (807071957) | Saccharomyces cerevisiae (Baker's yeast) | U4 snRNA |
| 2 | W | U6 snRNA | polymer | 112 | 35883.2 | 1 | GenBank (807071964) | Saccharomyces cerevisiae (Baker's yeast) | U6 snRNA |
| 3 | A | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) Pfam (PF08082) Pfam (PF08083) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Prp8 |
| 4 | H | U4/U6 small nuclear ribonucleoprotein PRP4 | polymer | 465 | 52507.0 | 1 | UniProt (P20053) Pfam (PF00400) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Pre-mRNA-processing protein 4, Prp4 |
| 5 | J | Pre-mRNA-splicing factor 6 | polymer | 899 | 104370.1 | 1 | UniProt (P19735) Pfam (PF06424) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Prp6 |
| 6 | D | Spliceosomal protein DIB1 | polymer | 143 | 16798.4 | 1 | UniProt (Q06819) Pfam (PF02966) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
| 7 | F | Pre-mRNA-processing factor 31 | polymer | 494 | 56382.5 | 1 | UniProt (P49704) Pfam (PF01798) Pfam (PF09785) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Prp31 |
| 8 | G | U4/U6 small nuclear ribonucleoprotein PRP3 | polymer | 469 | 55974.3 | 1 | UniProt (Q03338) Pfam (PF08572) Pfam (PF06544) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Pre-mRNA-splicing factor 3, Prp3 |
| 9 | B | Pre-mRNA-splicing helicase BRR2 | polymer | 2163 | 246470.3 | 1 | UniProt (P32639) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Protein Snu246, Brr2 |
| 10 | x | Unknown protein | polymer | 100 | 8528.5 | 1 | Saccharomyces cerevisiae (Baker's yeast) | ||
| 11 | k, b | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 12 | l, h | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Sm-D1,snRNP core protein D1, U4 SmD1 |
| 13 | m, j | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Sm-D2,snRNP core protein D2, SmD2 |
| 14 | n, d | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3, SmD3 |
| 15 | p, e | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 16 | q, f | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 17 | r, g | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 18 | E | Snu66 | polymer | 328 | 28660.0 | 1 | UniProt (by SIFTS) (Q12420) In PDB | Saccharomyces cerevisiae (Baker's yeast) | |
| 19 | U | U5 snRNA | polymer | 214 | 68643.3 | 1 | GenBank (807071959) | Saccharomyces cerevisiae (Baker's yeast) | |
| 20 | K | 13 kDa ribonucleoprotein-associated protein | polymer | 126 | 13582.9 | 1 | UniProt (P39990) Pfam (PF01248) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Small nuclear ribonucleoprotein-associated protein 1, Snu13 |
| 21 | 2 | U6 snRNA-associated Sm-like protein LSm2 | polymer | 95 | 11177.9 | 1 | UniProt (P38203) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Small nuclear ribonucleoprotein D homolog SNP3 |
| 22 | 3 | U6 snRNA-associated Sm-like protein LSm3 | polymer | 89 | 10039.3 | 1 | UniProt (P57743) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | SmX4 protein |
| 23 | 4 | U6 snRNA-associated Sm-like protein LSm4 | polymer | 187 | 21298.1 | 1 | UniProt (P40070) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | LSm4 |
| 24 | 5 | U6 snRNA-associated Sm-like protein LSm5 | polymer | 93 | 10433.0 | 1 | UniProt (P40089) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | LSm5 |
| 25 | 6 | U6 snRNA-associated Sm-like protein LSm6 | polymer | 86 | 9406.6 | 1 | UniProt (A6ZYX7) Pfam (PF01423) UniProt (by SIFTS) (Q06406) In PDB | Saccharomyces cerevisiae (Baker's yeast) | LSm6 |
| 26 | 7 | U6 snRNA-associated Sm-like protein LSm7 | polymer | 115 | 13027.0 | 1 | UniProt (P53905) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | LSm7 |
| 27 | 8 | U6 snRNA-associated Sm-like protein LSm8 | polymer | 109 | 12403.4 | 1 | UniProt (P47093) Pfam (PF01423) In PDB | Saccharomyces cerevisiae (Baker's yeast) | LSm8 |
| 28 | C | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114132.1 | 1 | UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679) In PDB | Saccharomyces cerevisiae (Baker's yeast) | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10, Snu114 |
| 29 | C | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) |
Sequence modifications
C: 1 - 1008 (UniProt: P36048)
| PDB | External Database | Details |
|---|---|---|
| Ser 530 | Glu 530 | conflict |
| Lys 531 | Asp 531 | conflict |
| Thr 532 | Asp 532 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 1403542.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 523.2 | |
| All* | Total formula weight | 1404066.1 |
