5GAN
The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Energy Filter | GIF Quantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Number of Particle | 473827 |
| Software | RELION,CTFFIND,Coot,REFMAC |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
