5G4W

S. enterica HisA mutant D7N, D10G, Dup13-15 (VVR) with substrate ProFAR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	HISA	polymer	256	27428.4	1	UniProt (V7IJE3) Pfam (PF00977) UniProt (by SIFTS) (P10372) In PDB	SALMONELLA ENTERICA
2	A	[(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate	non-polymer		577.3	1	Chemie (GUO)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4		water	water		18.0	69	Chemie (HOH)

Sequence modifications

A: 1 - 245 (UniProt: V7IJE3)

PDB	External Database	Details
Lys 246	-	expression tag
Gly 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
Asn 7	Asp 7	engineered mutation
Gly 10	Asp 10	engineered mutation
Val 13	-	insertion
Val 14	-	insertion
Arg 15	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27428.4
Non-Polymers*	Number of molecules	3
	Total formula weight	761.5
All*	Total formula weight	28189.9

*Water molecules are not included.