5G4E

S. enterica HisA mutant D10G, Dup13-15, Q24L, G102A, V106L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase	polymer	256	27442.4	2	UniProt (A0A630AQ07) Pfam (PF00977) UniProt (by SIFTS) (P10372)	Salmonella enterica	Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
2	C, H (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D, E, J, K (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
4	F, G (A, B)	ACETIC ACID	non-polymer		60.1	2	Chemie (ACY)
5	I (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	L, M (A, B)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A, B: 1 - 245 (UniProt: A0A630AQ07)

PDB	External Database	Details
Gly 10	Asp 10	engineered mutation
Val 15	-	insertion
Val 15	-	insertion
Arg 15	-	insertion
Leu 24	Gln 24	engineered mutation
Ala 102	Gly 102	engineered mutation
Leu 106	Val 106	engineered mutation
Lys 246	-	expression tag
Gly 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54884.9
Non-Polymers*	Number of molecules	9
	Total formula weight	496.3
All*	Total formula weight	55381.1

*Water molecules are not included.