5FQD

Structural basis of Lenalidomide induced CK1a degradation by the crl4crbn ubiquitin ligase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	DNA DAMAGE-BINDING PROTEIN 1	polymer	856	95773.7	2	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	HOMO SAPIENS (HUMAN)	DDB P127 SUBUNIT, DNA DAMAGE-BINDING PROTEIN A, DDBA, DAMAGE -SPECIFIC DNA-BINDING PROTEIN 1, HBV X-ASSOCIATED PROTEIN 1, XAP-1, UV-DAMAGED DNA-BINDING FACTOR, UV-DAMAGED DNA-BINDING PROTEIN 1, UV-DDB 1, XPE-BINDING FACTOR, XPE-BF, XERODERMA PIGMENTOSUM GROUP E-COMPLEMENTING PROTEIN, XPCE
2	B, E (B, E)	PROTEIN CEREBLON	polymer	426	48976.1	2	UniProt (Q96SW2) Pfam (PF02190) Pfam (PF03226)	HOMO SAPIENS (HUMAN)
3	C, F (C, F)	CASEIN KINASE I ISOFORM ALPHA	polymer	341	39302.2	2	UniProt (P48729) Pfam (PF00069)	HOMO SAPIENS (HUMAN)	CKI-ALPHA, CK1, CASEINE KINASE 1A
4	G, I (B, E)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	H, J (B, E)	S-Lenalidomide	non-polymer		259.3	2	Chemie (LVY)
6	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	305	Chemie (HOH)

Sequence modifications

A, D: 1 - 395 (UniProt: Q16531)

PDB	External Database	Details
Met -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Glu -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

A, D: 709 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly 396	-	linker
Asn 397	-	linker
Gly 398	-	linker
Asn 399	-	linker
Ser 400	-	linker
Gly 401	-	linker

B, E: 41 - 442 (UniProt: Q96SW2)

PDB	External Database	Details
Met 17	-	expression tag
Asp 18	-	expression tag
Trp 19	-	expression tag
Ser 20	-	expression tag
His 21	-	expression tag
Pro 22	-	expression tag
Gln 23	-	expression tag
Phe 24	-	expression tag
Glu 25	-	expression tag
Lys 26	-	expression tag
Ser 27	-	expression tag
Ala 28	-	expression tag
Val 29	-	expression tag
Asp 30	-	expression tag
Glu 31	-	expression tag
Asn 32	-	expression tag
Leu 33	-	expression tag
Tyr 34	-	expression tag
Phe 35	-	expression tag
Gln 36	-	expression tag
Gly 37	-	expression tag
Gly 38	-	expression tag
Gly 39	-	expression tag
Arg 40	-	expression tag

C, F: 1 - 337 (UniProt: P48729)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	368104.1
Non-Polymers*	Number of molecules	4
	Total formula weight	649.3
All*	Total formula weight	368753.4

*Water molecules are not included.