5FQ5
Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | SGRNA | polymer | 84 | 27118.2 | 1 | SYNTHETIC CONSTRUCT | ||
| 2 | B (B) | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 | polymer | 1372 | 158875.0 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | STREPTOCOCCUS PYOGENES | SPYCAS9 |
| 3 | C (C) | TARGET DNA STRAND PROXIMAL FRAGMENT | polymer | 11 | 3295.2 | 1 | SYNTHETIC CONSTRUCT | ||
| 4 | D (D) | NON-TARGET DNA STRAND | polymer | 11 | 3410.2 | 1 | SYNTHETIC CONSTRUCT | ||
| 5 | E (E) | TARGET DNA STRAND DISTAL FRAGMENT | polymer | 17 | 5215.4 | 1 | SYNTHETIC CONSTRUCT | ||
| 6 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | H, I, J, K, L... (A, B, D) | POTASSIUM ION | non-polymer | 39.1 | 13 | Chemie (K) | |||
| 8 | U, V, W, X, Y (A, B, C, D, E) | water | water | 18.0 | 1108 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 197914.1 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 556.9 | |
| All* | Total formula weight | 198471.0 |
