5FQ5
Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.740, 67.570, 188.190 |
Unit cell angles | 90.00, 111.31, 90.00 |
Refinement procedure
Resolution | 47.906 - 2.136 |
R-factor | 0.1915 |
Rwork | 0.191 |
R-free | 0.22480 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 0.657 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.140 | 1.500 |
Number of reflections | 113763 | |
<I/σ(I)> | 28.61 | 0.76 |
Completeness [%] | 96.7 | 65 |
Redundancy | 148.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.1 M TRIS-ACETATE PH 8.5, 0.3 M KSCN, 15% PEG (W/V) 3350 |