5FKV

cryo-EM structure of the E. coli replicative DNA polymerase complex bound to DNA (DNA polymerase III alpha, beta, epsilon, tau complex)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA POLYMERASE III SUBUNIT ALPHA	polymer	1160	130088.4	1	UniProt (P10443) Pfam (PF02811) Pfam (PF07733) Pfam (PF17657) Pfam (PF14579) Pfam (PF01336) Pfam (PF20914) In PDB	ESCHERICHIA COLI K-12
2	B, C	DNA POLYMERASE III BETA	polymer	366	40630.5	2	UniProt (P0A988) Pfam (PF00712) Pfam (PF02767) Pfam (PF02768) In PDB	ESCHERICHIA COLI K-12
3	D	DNA POLYMERASE III EPSILON	polymer	243	27119.0	1	UniProt (P03007) Pfam (PF00929) In PDB	ESCHERICHIA COLI K-12
4	E	DNA POLYMERASE III TAU	polymer	144	16284.3	1	UniProt (P06710) Pfam (PF12170) In PDB	ESCHERICHIA COLI K-12
5	P	PRIMER-TEMPLATE DUPLEX DNA	polymer	25	7797.0	1		SYNTHETIC CONSTRUCT
6	T	PRIMER-TEMPLATE DUPLEX DNA	polymer	29	8796.7	1		SYNTHETIC CONSTRUCT

Sequence modifications

A: 1 - 1160 (UniProt: P10443)

PDB	External Database	Details
Leu 921	Ala 921	engineered mutation
Leu 923	Met 923	engineered mutation

D: 1 - 243 (UniProt: P03007)

PDB	External Database	Details
Leu 183	Thr 183	engineered mutation
Leu 185	Met 185	engineered mutation
Pro 186	Ala 186	engineered mutation
Leu 187	Phe 187	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	271346.4
All*	Total formula weight	271346.4

*Water molecules are not included.