5FKV

cryo-EM structure of the E. coli replicative DNA polymerase complex bound to DNA (DNA polymerase III alpha, beta, epsilon, tau complex)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003824	molecular_function	catalytic activity
A	0003887	molecular_function	DNA-directed DNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006260	biological_process	DNA replication
A	0006261	biological_process	DNA-templated DNA replication
A	0006272	biological_process	leading strand elongation
A	0006273	biological_process	lagging strand elongation
A	0008408	molecular_function	3'-5' exonuclease activity
A	0009360	cellular_component	DNA polymerase III complex
A	0030894	cellular_component	replisome
A	0044776	cellular_component	DNA polymerase III, core complex
A	0071897	biological_process	DNA biosynthetic process
B	0003677	molecular_function	DNA binding
B	0003887	molecular_function	DNA-directed DNA polymerase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0006271	biological_process	DNA strand elongation involved in DNA replication
B	0006974	biological_process	DNA damage response
B	0008408	molecular_function	3'-5' exonuclease activity
B	0009360	cellular_component	DNA polymerase III complex
B	0030174	biological_process	regulation of DNA-templated DNA replication initiation
B	0030894	cellular_component	replisome
B	0032297	biological_process	negative regulation of DNA-templated DNA replication initiation
B	0042276	biological_process	error-prone translesion synthesis
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0044787	biological_process	bacterial-type DNA replication
B	1990078	cellular_component	replication inhibiting complex
B	1990085	cellular_component	Hda-beta clamp complex
C	0003677	molecular_function	DNA binding
C	0003887	molecular_function	DNA-directed DNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006261	biological_process	DNA-templated DNA replication
C	0006271	biological_process	DNA strand elongation involved in DNA replication
C	0006974	biological_process	DNA damage response
C	0008408	molecular_function	3'-5' exonuclease activity
C	0009360	cellular_component	DNA polymerase III complex
C	0030174	biological_process	regulation of DNA-templated DNA replication initiation
C	0030894	cellular_component	replisome
C	0032297	biological_process	negative regulation of DNA-templated DNA replication initiation
C	0042276	biological_process	error-prone translesion synthesis
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0044787	biological_process	bacterial-type DNA replication
C	1990078	cellular_component	replication inhibiting complex
C	1990085	cellular_component	Hda-beta clamp complex
D	0003676	molecular_function	nucleic acid binding
D	0003677	molecular_function	DNA binding
D	0003887	molecular_function	DNA-directed DNA polymerase activity
D	0004527	molecular_function	exonuclease activity
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006259	biological_process	DNA metabolic process
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006272	biological_process	leading strand elongation
D	0006273	biological_process	lagging strand elongation
D	0008408	molecular_function	3'-5' exonuclease activity
D	0009360	cellular_component	DNA polymerase III complex
D	0030894	cellular_component	replisome
D	0044776	cellular_component	DNA polymerase III, core complex
D	0045004	biological_process	DNA replication proofreading
D	0046872	molecular_function	metal ion binding
D	0071897	biological_process	DNA biosynthetic process
E	0003887	molecular_function	DNA-directed DNA polymerase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
D	HIS162
B	GLN149
B	TYR153
C	ARG24
C	GLN149
C	TYR153

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site_id	SWS_FT_FI2
Number of Residues	5
Details	BINDING:

Chain	Residue	Details
D	ASP12
D	GLU14
D	GLU61
D	HIS66
D	ASP167

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 754

Chain	Residue	Details
D	ASP12	metal ligand
D	GLU14	hydrogen bond acceptor, metal ligand, proton acceptor, proton donor
D	GLU61	electrostatic stabiliser, increase basicity, polar interaction
D	HIS162	proton acceptor, proton donor
D	ASP167	metal ligand

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