5FER

Complex of TRIM25 RING with UbcH5-Ub

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	E3 ubiquitin/ISG15 ligase TRIM25	polymer	85	9276.7	2	UniProt (Q14258) Pfam (PF13445)	Homo sapiens (Human)	Estrogen-responsive finger protein,RING finger protein 147,RING-type E3 ubiquitin transferase,Tripartite motif-containing protein 25,Ubiquitin/ISG15-conjugating enzyme TRIM25,Zinc finger protein 147
2	B, E (B, E)	Ubiquitin-conjugating enzyme E2 D1	polymer	150	16929.4	2	UniProt (P51668) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D1,E2 ubiquitin-conjugating enzyme D1,Stimulator of Fe transport,SFT,UBC4/5 homolog,UbcH5,Ubiquitin carrier protein D1,Ubiquitin-conjugating enzyme E2(17)KB 1,Ubiquitin-conjugating enzyme E2-17 kDa 1,Ubiquitin-protein ligase D1
3	C, F (C, F)	Ubiquitin-40S ribosomal protein S27a	polymer	76	8576.8	2	UniProt (P62992) Pfam (PF00240)	Bos taurus (Bovine)	Ubiquitin carboxyl extension protein 80
4	G, H, I, J (A, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	68	Chemie (HOH)

Sequence modifications

A, D: 1 - 82 (UniProt: Q14258)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

B, E: 1 - 147 (UniProt: P51668)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	69565.7
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	69827.4

*Water molecules are not included.