5FER
Complex of TRIM25 RING with UbcH5-Ub
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.2829 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.480, 71.700, 160.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.295 - 2.340 |
R-factor | 0.2293 |
Rwork | 0.227 |
R-free | 0.27310 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.539 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.280 | 2.420 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.139 | 1.918 |
Number of reflections | 30000 | |
<I/σ(I)> | 13.75 | |
Completeness [%] | 99.3 | |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 10% PEG 20000 and 100 mM Tris pH 8.5 |