5FER

Complex of TRIM25 RING with UbcH5-Ub

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000166	molecular_function	nucleotide binding
B	0000209	biological_process	protein polyubiquitination
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0030514	biological_process	negative regulation of BMP signaling pathway
B	0031398	biological_process	positive regulation of protein ubiquitination
B	0032991	cellular_component	protein-containing complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0061631	molecular_function	ubiquitin conjugating enzyme activity
B	0070936	biological_process	protein K48-linked ubiquitination
B	1904262	biological_process	negative regulation of TORC1 signaling
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0000166	molecular_function	nucleotide binding
E	0000209	biological_process	protein polyubiquitination
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0030514	biological_process	negative regulation of BMP signaling pathway
E	0031398	biological_process	positive regulation of protein ubiquitination
E	0032991	cellular_component	protein-containing complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0061631	molecular_function	ubiquitin conjugating enzyme activity
E	0070936	biological_process	protein K48-linked ubiquitination
E	1904262	biological_process	negative regulation of TORC1 signaling

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue ZN A 101

Chain	Residue
A	CYS13
A	CYS16
A	LYS21
A	CYS33
A	CYS36

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 102

Chain	Residue
A	CYS28
A	HIS30
A	CYS50
A	CYS53

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN D 101

Chain	Residue
D	CYS28
D	HIS30
D	CYS50
D	CYS53

---

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN D 102

Chain	Residue
D	CYS13
D	CYS16
D	CYS33
D	CYS36

---

site_id	AC5
Number of Residues	13
Details	binding site for Di-peptide GLY F 76 and LYS E 85

Chain	Residue
E	HIS75
E	ASN77
E	ILE78
E	ILE84
E	LEU86
E	ASP117
E	PRO118
E	LEU119
E	VAL120
E	ILE123
E	TYR134
F	ARG74
F	GLY75

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
C	LYS27-ASP52

---

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. CgHnFCgsCL

Chain	Residue	Details
A	CYS28-LEU37

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	82
Details	Zinc finger: {"description":"RING-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00175","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PDB","id":"5EYA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5FER","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10133","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	150
Details	Domain: {"description":"Ubiquitin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Site: {"description":"Interacts with activating enzyme"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Site: {"description":"Essential for function"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by PINK1","evidences":[{"source":"UniProtKB","id":"P62979","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"ADP-ribosylglycine","evidences":[{"source":"UniProtKB","id":"P62979","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	14
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P62979","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)"}

Chain

Residue

Details

---