5F4E

Crystal structure of the human sperm Izumo1 and egg Juno complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Izumo sperm-egg fusion protein 1	polymer	239	27139.2	1	UniProt (Q8IYV9) Pfam (PF15005) Pfam (PF16706) In PDB	Homo sapiens (Human)	Oocyte binding/fusion factor,OBF,Sperm-specific protein izumo
2	B	Sperm-egg fusion protein Juno	polymer	215	24575.9	1	UniProt (A6ND01) Pfam (PF03024) In PDB	Homo sapiens (Human)	Folate receptor 4,Folate receptor delta,FR-delta,IZUMO1 receptor protein JUNO
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	25	Chemie (HOH)

Sequence modifications

A: 22 - 254 (UniProt: Q8IYV9)

PDB	External Database	Details
Gly 255	-	expression tag
Arg 256	-	expression tag
Ala 257	-	expression tag
Val 258	-	expression tag
Pro 259	-	expression tag
Arg 260	-	expression tag

B: 20 - 228 (UniProt: A6ND01)

PDB	External Database	Details
Gly 229	-	expression tag
Arg 230	-	expression tag
Leu 231	-	expression tag
Val 232	-	expression tag
Pro 233	-	expression tag
Arg 234	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51715.1
Non-Polymers*	Number of molecules	3
	Total formula weight	348.8
All*	Total formula weight	52063.8

*Water molecules are not included.