5F4E

Crystal structure of the human sperm Izumo1 and egg Juno complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-24
Detector	RAYONIX MX-300
Wavelength(s)	1.77
Spacegroup name	C 1 2 1
Unit cell lengths	148.000, 55.800, 81.600
Unit cell angles	90.00, 103.60, 90.00

Refinement procedure

Resolution	47.962 - 2.400
R-factor	0.1822
Rwork	0.180
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lrh
RMSD bond length	0.010
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.962	47.960	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.076	0.055	0.679
Rpim	0.030	0.023	0.270
Total number of observations	184910	3469	18998
Number of reflections	25587
<I/σ(I)>	14.4	33.6	2.8
Completeness [%]	100.0	99.4	99.8
Redundancy	7.2	6.6	7.2
CC(1/2)	0.998	0.996	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M MES [pH 6.5], 20% (w/v) PEG 4000 and 0.6 M NaCl