5ERG

Crystal structure of the two-subunit tRNA m1A58 methyltransferase TRM6-TRM61 in complex with SAM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	polymer	488	55720.5	1	UniProt (P41814) Pfam (PF04189)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	General control non-derepressible protein 10,Protein GCD10,tRNA(m1A58)-methyltransferase subunit TRM6,tRNA(m1A58)MTase subunit TRM6
2	B (B)	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61	polymer	383	43987.7	1	UniProt (P46959) Pfam (PF08704)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	General control non-derepressible protein 14,Protein GCD14,tRNA(m1A58)-methyltransferase subunit TRM61,tRNA(m1A58)MTase subunit TRM61
3	C (B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
4	D, E (A, B)	water	water		18.0	199	Chemie (HOH)

Sequence modifications

A: 1 - 478 (UniProt: P41814)

PDB	External Database	Details
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99708.2
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	100106.6

*Water molecules are not included.