5ERG
Crystal structure of the two-subunit tRNA m1A58 methyltransferase TRM6-TRM61 in complex with SAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 | polymer | 488 | 55720.5 | 1 | UniProt (P41814) Pfam (PF04189) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | General control non-derepressible protein 10,Protein GCD10,tRNA(m1A58)-methyltransferase subunit TRM6,tRNA(m1A58)MTase subunit TRM6 |
| 2 | B (B) | tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 | polymer | 383 | 43987.7 | 1 | UniProt (P46959) Pfam (PF08704) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | General control non-derepressible protein 14,Protein GCD14,tRNA(m1A58)-methyltransferase subunit TRM61,tRNA(m1A58)MTase subunit TRM61 |
| 3 | C (B) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) | |||
| 4 | D, E (A, B) | water | water | 18.0 | 199 | Chemie (HOH) |
Sequence modifications
A: 1 - 478 (UniProt: P41814)
| PDB | External Database | Details |
|---|---|---|
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 99708.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 398.4 | |
| All* | Total formula weight | 100106.6 |
