5ERG

Crystal structure of the two-subunit tRNA m1A58 methyltransferase TRM6-TRM61 in complex with SAM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	polymer	488	55720.5	1	UniProt (P41814) Pfam (PF04189) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	General control non-derepressible protein 10,Protein GCD10,tRNA(m1A58)-methyltransferase subunit TRM6,tRNA(m1A58)MTase subunit TRM6
2	B	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61	polymer	383	43987.7	1	UniProt (P46959) Pfam (PF08704) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	General control non-derepressible protein 14,Protein GCD14,tRNA(m1A58)-methyltransferase subunit TRM61,tRNA(m1A58)MTase subunit TRM61
3	B	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
4		water	water		18.0	199	Chemie (HOH)

Sequence modifications

A: 1 - 478 (UniProt: P41814)

PDB	External Database	Details
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99708.2
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	100106.6

*Water molecules are not included.