5EOA

Crystal structure of OPTN E50K mutant and TBK1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Optineurin	polymer	82	9651.0	2	UniProt (Q96CV9) Pfam (PF11577) In PDB	Homo sapiens (Human)	E3-14.7K-interacting protein,FIP-2,Huntingtin yeast partner L,Huntingtin-interacting protein 7,HIP-7,Huntingtin-interacting protein L,NEMO-related protein,Optic neuropathy-inducing protein,Transcription factor IIIA-interacting protein,TFIIIA-IntP
2	C, D	Serine/threonine-protein kinase TBK1	polymer	57	6247.2	2	UniProt (Q9UHD2) In PDB	Homo sapiens (Human)	NF-kappa-B-activating kinase,T2K,TANK-binding kinase 1
3		water	water		18.0	24	Chemie (HOH)

Sequence modifications

A, B: 26 - 103 (UniProt: Q96CV9)

PDB	External Database	Details
Gly 22	-	expression tag
Pro 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
Lys 50	Glu 50	engineered mutation

C, D: 677 - 729 (UniProt: Q9UHD2)

PDB	External Database	Details
Gly 673	-	expression tag
Pro 674	-	expression tag
Gly 675	-	expression tag
Ser 676	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	31796.2
All*	Total formula weight	31796.2

*Water molecules are not included.