5EOA
Crystal structure of OPTN E50K mutant and TBK1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 291.15 |
Detector technology | CCD |
Collection date | 2015-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.824, 54.549, 153.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.444 - 2.503 |
R-factor | 0.2018 |
Rwork | 0.199 |
R-free | 0.26680 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.896 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 11226 |
<I/σ(I)> | 10.42 |
Completeness [%] | 99.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 291.5 | 0.1M MES monohydrate pH 6.0, 14% w/V Polyethylene glycol 4000 |